Catalogue number
CYT-929
Synonyms
Erythropoietin Receptor, EPO-R, EPOR.
Introduction
Erythropoietin
receptor, also known as EPOR arbitrates erythropoietin-induced erythroblast
proliferation as well as differentiation. During EPO binding, EPOR activates Jak2
tyrosine kinase which activates various intracellular pathways including:
Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors.
Furthermore, stimulated EPOR has a function in erythroid cell survival. Mutations
in EPOR may possibly produce erythroleukemia and familial erythrocytosis. In
addition, dysregulation of EPOR can affect on the growth of selected tumors.
Description
EPOR Human Recombinant produced in Sf9 Baculovirus cells is
a single, glycosylated polypeptide chain containing 232 amino acids (25-250 a.a)
and having a molecular mass of 25.6kDa. (Migrates at 28-40kDa on SDS-PAGE under
reducing conditions). EPOR is fused to an 6 amino acid His-tag at C-terminus
& purified by proprietary chromatographic techniques.
Source
Sf9,
Baculovirus cells.
Physical Appearance
Sterile Filtered clear solution.
Formulation
EPOR protein solution (0.5mg/ml) containing Phosphate
Buffered Saline (pH 7.4) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater
than 95.0% as determined by SDS-PAGE.
Amino acid sequence
APPPNLPDPK FESKAALLAA RGPEELLCFT ERLEDLVCFW EEAASAGVGP
GNYSFSYQLE DEPWKLCRLH QAPTARGAVR FWCSLPTADT SSFVPLELRV TAASGAPRYH RVIHINEVVL
LDAPVGLVAR LADESGHVVL RWLPPPETPM TSHIRYEVDV SAGNGAGSVQ RVEILEGRTE CVLSNLRGRT RYTFAVRARM
AEPSFGGFWS AWSEPVSLLT PSDLDPHHHH HH.
Safety Data Sheet
Usage
ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
