Catalogue number
PRO-2010
Synonyms
SH3 and cysteine rich domain, STAC, STAC1, SH3 and cysteine-rich domain-containing protein, Src homology 3 and cysteine-rich domain-containing protein.
Introduction
SH3 and Cysteine Rich Domain, which is also known as STAC, holds one phorbol-ester/DAG-type zinc finger and one SH3 domain. STAC takes part in a neuron-specific signal transduction.
Description
STAC Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 425 amino acids (1-402 a.a.) and having a molecular mass of 46.9kDa.
STAC is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
STAC protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMIPPSSP REDGVDGLPK EAVGAEQPPS PASTSSQESK LQKLKRSLSF KTKSLRSKSA DNFFQRTNSE DMKLQAHMVA EISPSSSPLP APGSLTSTPA RAGLHPGGKA HAFQEYIFKK PTFCDVCNHM IVGTNAKHGL RCKACKMSIH HKCTDGLAPQ RCMGKLPKGF RRYYSSPLLI HEQFGCIKEV MPIACGNKVD PVYETLRFGT SLAQRTKKGS SGSGSDSPHR TSTSDLVEVP EEANGPGGGY DLRKRSNSVF TYPENGTDDF RDPAKNINHQ GSLSKDPLQM NTYVALYKFV PQENEDLEMR PGDIITLLED SNEDWWKGKI QDRIGFFPAN FVQRLQQNEK IFRCVRTFIG CKEQGQITLK ENQICVSSEE EQDGFIRVLS GKKKGLIPLD VLENI.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
