Catalogue number
PRO-2074
Synonyms
PCI Domain Containing 2, CSN12-Like Protein, PCI
Domain-Containing Protein 2, F10, PCI domain-containing protein 2.
Introduction
PCI
Domain Containing 2 (PCID2) is expressed in immature and early-stage B
lymphocytes and regulates the expression of mitotic checkpoint protein MAD2. PCID2
is a component of the TREX-2 complex, transcription and export complex 2, this
complex functions in docking export-competent ribonucleoprotein particles
(mRNPs) to the nuclear entrance of the nuclear pore complex, nuclear basket.
Description
PCID2 Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 341 amino acids (80-399 a.a) and
having a molecular mass of 39.3kDa.
PCID2 is fused to a 21 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
E.coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
PCID2 protein solution (1.0 mg/ml) containing 20mM
Tris-HCl buffer (pH 8.0), 10% glycerol and 0.4M Urea.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MAYKCQTVIV QSFLRAFQAH KEENWALPVM YAVALDLRVF ANNADQQLVK KGKSKVGDML
EKAAELLMSC FRVCASDTRA GIEDSKKWGM LFLVNQLFKI YFKINKLHLC KPLIRAIDSS NLKDDYSTAQ
RVTYKYYVGR KAMFDSDFKQ AEEYLSFAFE
HCHRSSQKNK RMILIYLLPV KMLLGHMPTV ELLKKYHLMQ FAEVTRAVSE
GNLLLLHEAL AKHEAFFIRC GIFLILEKLK IITYRNLFKK VYLLLKTHQL SLDAFLVALK FMQVEDVDID
EVQCILANLI YMGHVKGYIS HQHQKLVVSK QNPFPPLSTV C.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
