Catalogue number
PRO-2313
Synonyms
Meprin
A subunit beta (EC:3.4.24.63), Endopeptidase-2, Meprin B, Mep1b, Mep-1b.
Introduction
Meprin A subunit beta (MEP1B) belongs to the astacin family of zinc endopeptidases 1, 2. MEP1B is a disulfide-linked, tetrameric metalloendopeptidase in renal brush border membranes. MEP1B is highly regulated, secreted cell-surface metalloendopeptidase, which is amply expressed in the kidney and intestine.
Description
MEP1B produced in Sf9
Insect cells is a single, glycosylated polypeptide chain containing 642 amino
acids (21-654aa) and having a molecular mass of 72.6kDa. (Molecular size on
SDS-PAGE will appear at approximately 70-100kDa).
MEP1Bis expressed with an
8 amino acid His tag at C-Terminus and purified by proprietary
chromatographic techniques.
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Source
Sf9 Insect cells.
Physical Appearance
Sterile filtered colorless solution.
Formulation
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MEP1B protein solution (0.25mg/ml)
contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
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Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as
determined by SDS-PAGE.
Amino acid sequence
LPAPEKFVKD
IDGGIDQDIF DINQGLGLDL FEGDIKLEAN GKNSIIGDHK RWPHTIPYVL EDSLEMNAKG VILNAFERYR
LKTCIDFKPW SGEANYISVF KGSGCWSSVG NIHAGKQELS IGTNCDRIAT VQHEFLHALG FWHEQSRADR
DDYVIIVWDR IQPGKEHNFN IYNDSVSDSL NVPYDYTSVM HYSKTAFQNG TESTIVTRIS EFEDVIGQRM
DFSDYDLLKL NQLYNCTSSL SFMDSCDFEL ENICGMIQSS GDSADWQRVS QVLSGPESDH SKMGQCKDSG
FFMHFNTSIL NEGATAMLES RLLYPKRGFQ CLEFYLYNSG SGNDQLNIYT REYTTGQQGG VLTLQRQIKE
VPIGSWQLHY VTLQVTKKFR VVFEGLRGPG TSSGGLSIDD INLSETRCPH HIWHIQNFTQ ILGGQDTSVY
SPPFYSSKGY AFQIYMDLRS STNVGIYFHL ISGANDDQLQ WPCPWQQATM TLLDQNPDIR QRMFNQRSIT
TDPTMTSDNG SYFWDRPSKV GVTDVFPNGT QFSRGIGYGT TVFITRERLK SREFIKGDDI YILLTVEDIS
HLNSTSAVPD PVPTLAVHNA CSEVVCQNGG ICVVQDGRAE CKCPAGEDWW YMGKRCEKRG STRDVEHHHH
HH.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
