Catalogue number
PRO-1889
Synonyms
Major Histocompatibility Complex, Class II, DR Alpha, HLA-DRA1,
MHC Class II AntigenDRA, MLRW, Histocompatibility AntigenHLA-DR
Alpha, HLAClass II Histocompatibility Antigen, DR Alpha Chain, MHC Cell
Surface Glycoprotein, HLA-DRA1.
Introduction
Major histocompatibility complex, class II, DR alpha (HLA-DRA) belongs
to the MHC class II family. HLA-DRA binds peptides derived from antigens which
access the endocytic route of antigen presenting cells (APC) and presents them
on the cell surface for identification by the CD4 T-cells. The peptide binding
cleft accommodates peptides of 10-30 residues. The peptides presented by MHC
class II molecules are generated mainly by degradation of proteins which access
the endocytic route, where they are processed by lysosomal proteases and other
hydrolases.
Description
HLA-DRA Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 212 amino acids (26-216 a.a) and
having a molecular mass of 24.3kDa.
HLA-DRA is fused to a 21 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
HLA-DRA protein solution (0.5mg/ml) containing 20mM Tris-HCl
buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze thaw cycles.
Purity
Greater
than 90% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MIKEEHVIIQ AEFYLNPDQS GEFMFDFDGD EIFHVDMAKK ETVWRLEEFG RFASFEAQGA
LANIAVDKAN LEIMTKRSNY TPITNVPPEV TVLTNSPVEL REPNVLICFI DKFTPPVVNV TWLRNGKPVT
TGVSETVFLP REDHLFRKFH YLPFLPSTED VYDCRVEHWG LDEPLLKHWE FDAPSPLPET TE
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
