Catalogue number
ENZ-626
Synonyms
Ubiquitin-conjugating enzyme E2 J2, ubiquitin-conjugating enzyme E2 J2 (UBC6 homolog east), Non-canonical ubiquitin-conjugating enzyme 2, ubiquitin conjugating enzyme 6, PRO2121, yeast UBC6 homolog, Ubc6p, NCUBE-2, EC 6.3.2.19.
Introduction
Ubiquitin-conjugating enzyme E2 J2 (UBE2J2) is a member of the ubiquitin-conjugating enzyme family. UBE2J2 catalyzes the covalent attachment of ubiquitin to other proteins. UBE2J2 is located in the membrane of the endoplasmic reticulum. UBE2J2 participates in the selective degradation of misfolded membrane proteins from the endoplasmic reticulum (ERAD).
Description
UBE2J2 Human Recombinant produced in E. coli is a single polypeptide chain containing 250 amino acids (1-226) and having a molecular mass of 28.0 kDa.
UBE2J2 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
E.coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
The UBE2J2 solution (1mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 200mM NaCl, 1mM DTT and 30% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSHMSSTSS KRAPTTATQR LKQDYLRIKK DPVPYICAEP LPSNILEWHY VVRGPEMTPY EGGYYHGKLI FPREFPFKPP SIYMITPNGR FKCNTRLCLS ITDFHPDTWN PAWSVSTILT GLLSFMVEKG PTLGSIETSD FTKRQLAVQS LAFNLKDKVF CELFPEVVEE IKQKQKAQDE LSSRPQTLPL PDVVPDGETH LVQNGIQLLN GHAPGAVPNL AGLQQANRHH
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
