Catalogue number
ENZ-956
Synonyms
POGLUT1, C3orf9, CLP46, hCLP46, KDELCL1, KTELC1, Protein
O-glucosyltransferase 1, CAP10-like 46 kDa protein, KTEL motif-containing
protein 1, Myelodysplastic syndromes relative protein, O-glucosyltransferase
Rumi homolog, hRumi, Protein O-xylosyltransferase, MDSRP.
Introduction
POGLUT1 is a homologue of Rumi from
Drosophila, an endoplasmic reticulum (ER)-retaining glucosyltransferase which catalyzes
the transfer of glucose and xylose from UDP-glucose and UDP-xylose,
respectively, to EGF repeats on the consensus sequence C-X-S-X-P-C. POGLUT1 positively
regulates Notch signaling without affecting Notch ligand binding.
Description
POGLUT1 Human Recombinant produced in in Sf9 Baculovirus
cells is a single, non-glycosylated polypeptide chain containing 377 amino
acids (24-392a.a) and having a molecular mass of 44.5kDa (Migrates at 40-57kDa
on SDS-PAGE under reducing conditions).
POGLUT1 is fused to a 8 amino acid His-tag at C-Terminus
and purified by proprietary chromatographic techniques.
Source
Sf9,
Baculovirus cells.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
The POGLUT1 solution (0.25mg/ml) contains Phosphate
Buffered Saline (pH 7.4) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90% as determined by SDS-PAGE.
Amino acid sequence
RQKESGSKWK
VFIDQINRSL ENYEPCSSQN CSCYHGVIEE DLTPFRGGIS RKMMAEVVRR KLGTHYQITK NRLYRENDCM
FPSRCSGVEH FILEVIGRLP DMEMVINVRD YPQVPKWMEP AIPVFSFSKT SEYHDIMYPA WTFWEGGPAV
WPIYPTGLGR WDLFREDLVR SAAQWPWKKK NSTAYFRGSR TSPERDPLIL LSRKNPKLVD AEYTKNQAWK
SMKDTLGKPA AKDVHLVDHC KYKYLFNFRG VAASFRFKHL FLCGSLVFHV GDEWLEFFYP QLKPWVHYIP
VKTDLSNVQE LLQFVKANDD VAQEIAERGS QFIRNHLQMD DITCYWENLL SEYSKFLSYN VTRRKGYDQI
IPKMLKTELL EHHHHHH.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
