Catalogue number
ENZ-177
Synonyms
Peroxisomal trans-2-enoyl-CoA reductase, TERP, HSA250303, SDR29C1, 2,4-dienoyl-CoA reductase-related protein, DCR-RP, pVI-ARL, EC 1.3.1.38, HPDHASE, putative short chain alcohol dehydrogenase, short chain dehydrogenase/reductase family 29C member 1.
Introduction
PECR is the main enzyme for a projected peroxisomal chain elongation pathway and is primarily expressed in kidney and liver.
Description
PECR Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 327 amino acids (1-303) and having a molecular mass of 35.1 kDa.
PECR is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
E.coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
The PECR solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 2mM DTT and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 95% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSHMASWAK GRSYLAPGLL QGQVAIVTGG ATGIGKAIVK ELLELGSNVV IASRKLERLK SAADELQANL PPTKQARVIP IQCNIRNEEE VNNLVKSTLD TFGKINFLVN NGGGQFLSPA EHISSKGWHA VLETNLTGTF YMCKAVYSSW MKEHGGSIVN IIVPTKAGFP LAVHSGAARA GVYNLTKSLA LEWACSGIRI NCVAPGVIYS QTAVENYGSW GQSFFEGSFQ KIPAKRIGVP EEVSSVVCFL LSPAASFITG QSVDVDGGRS LYTHSYEVPD HDNWPKGAGD LSVVKKMKET FKEKAKL
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
