Catalogue number
ANT-331
Synonyms
ALK/P80 Protein, ALK/P80, p80 protein, anaplastic lymphoma kinase, ALK, nucleophosmin, NPM.
Type
Mouse Anti Human Monoclonal.
Introduction
ALK/p80 is a human p80 protein, identified as a hybrid of the anaplastic lymphoma kinase (ALK) gene and the nucleophosmin (NPM) gene resulting from the t(2;5)(p23;q35) translocation found in a third of large cell lymphomas.
Clone
P5E3AT.
Immunogen
Anti-human ALK/P80 mAb is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human ALK/P80 amino acids 81-294 purified from E. coli.
Ig Subclass
Mouse IgG2b heavy chain and κ light chain.
Purification Method
ALK/P80 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Formulation
1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.
Storage Procedures
For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C.
Prevent freeze thaw cycles.
Stability / Shelf Life
12 months at -20°C. 1 month at 4°C.
Applications
ALK/P80 antibody has been tested by ELISA, Western blot and Immunohistochemical analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 2,000. Recommended starting dilution is 1:1,000.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
