Catalogue number
ANT-567
Synonyms
Fructose-bisphosphate aldolase A, Muscle-type aldolase, Lung cancer antigen NY-LU-1, ALDOA, ALDA, EC 4.1.2.13, GSD12, MGC10942, MGC17716, MGC17767, Aldolase-A.
Type
Mouse Anti Human Monoclonal.
Introduction
Aldolase A (ALDOA) is a glycolytic enzyme, which catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. ALDOA is found in the developing embryo and is produced in even greater amounts in ***** muscle. ALDOA expression is repressed in the ***** liver, kidney and intestine and similar to ALDOC levels in the brain and other nervous tissue. ALDOA deficiency has been linked with myopathy and hemolytic anemia.
Clone
PAT3F9AT.
Physical Appearance
Sterile filtered colorless solution.
Immunogen
Anti-human ALDOA mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human ALDOA amino acids 1-364 purified from E. coli.
Ig Subclass
Mouse IgG2a heavy chain and?k light chain.
Purification Method
ALDOA antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
Formulation
1mg/ml containing PBS, pH-7.4, 10% Glycerol and 0.02% Sodium Azide.
Storage Procedures
For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C.?
Prevent freeze thaw cycles.
Stability / Shelf Life
12 months at -20°C. 1 month at 4°C.
Applications
ALDOA antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1000. Recommended dilution range for ICC/IF and Flow cytometry is 1:200.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
