Catalogue number
PRO-2350
Synonyms
Tyrosinase Related Protein 1, Tyrosinase-Related Protein
1, Melanoma Antigen Gp75, Glycoprotein 75, DHICA Oxidase, Catalase B, CAS2, TRP1,
TYRP, TRP, 5,6-Dihydroxyindole-2-Carboxylic Acid Oxidase, EC 1.14.18.1, EC
1.14.18.-, EC 1.14.18, B-PROTEIN, TRP-1, TYRRP, CATB, GP75, OCA3, 5,6-dihydroxyindole-2-carboxylic
acid oxidase.
Introduction
TYRP1,
also known as 5, 6-dihydroxyindole-2-carboxylic acid oxidase, is a melanosomal
enzyme which is a member of the tyrosinase family and takes a significant part
in the melanin biosynthetic pathway. TYRP1 is a melanocyte-specific
gene which is involved in eumelanin synthesis. Furthermore, TYRP1 is implicated
in the oxidation of 5,6-dihydroxyindole-2-carboxylic
acid-DHICA into indole-5,6-quinone-2-carboxylic acid. TYRP1 is regulated by the
microphthalmia-associated transcription
factor-MITF.
Description
TYRP1 Human Recombinant produced in Sf9 Baculovirus cells
is a single, non-glycosylated polypeptide chain containing 462 amino acids (25-477a.a)
and having a molecular mass of 52.5kDa. (Molecular size on SDS-PAGE will appear
at approximately 50-70kDa).
TYRP1 is fused to a 6 amino acid His-tag at C-terminus
& purified by proprietary chromatographic techniques.
Source
Sf9,
Baculovirus cells.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
TYRP1 protein solution (0.25mg/ml) containing Phosphate
Buffered Saline (pH 7.4) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Amino acid sequence
ADLQFPRQCA
TVEALRSGMC CPDLSPVSGP GTDRCGSSSG RGRCEAVTAD SRPHSPQYPH DGRDDREVWP LRFFNRTCHC
NGNFSGHNCG TCRPGWRGAA CDQRVLIVRR NLLDLSKEEK NHFVRALDMA KRTTHPLFVI ATRRSEEILG
PDGNTPQFEN ISIYNYFVWT HYYSVKKTFL
GVGQESFGEV DFSHEGPAFL TWHRYHLLRL EKDMQEMLQE PSFSLPYWNF
ATGKNVCDIC TDDLMGSRSN FDSTLISPNS VFSQWRVVCD SLEDYDTLGT LCNSTEDGPI RRNPAGNVAR
PMVQRLPEPQ DVAQCLEVGL FDTPPFYSNS TNSFRNTVEG YSDPTGKYDP AVRSLHNLAH
LFLNGTGGQT HLSPNDPIFV
LLHTFTDAVF DEWLRRYNAD ISTFPLENAP IGHNRQYNMV PFWPPVTNTE MFVTAPDNLG YTYEIQWPSR EFSVPEHHHH
HH.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
