Catalogue number
CYT-315
Synonyms
gp55, HLA-2, L3 / T4, Ly-4, T cell antigen T4/LEU3, T4, sCD4.
Introduction
CD4, a single chain transmembrane glycoprotein, is found on a T cell subset (helper/inducer) representing 45% of peripheral blood lymphocytes. It is also present on 80% of thymocytes and at a lower level on monocytes. It is involved in recognition of antigen presented along with MHC class II by APCs. It serves as receptor for HIV. Antibody to CD4 recognizes T-helper cells required for recognition of class II antigens. It reacts with ~60% of peripheral blood E rosette-positive (E+) cells while showing negligible reactivity with E- cells, monocytes, granulocytes, EBV-transformed B cell lines, and mouse splenocytes.
Description
CD-4 Human Recombinant is fused with a 4kDa His Tag and encoding amino acids 125-202, having a total molecular weight of 19 kDa.
Source
Escherichia Coli.
Physical Appearance
Sterile lyophilized powder.
Formulation
Each mg contains 1X PBS.
Solubility
It is recommended to reconstitute the lyophilized CD-4 (125-202) in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
Stability
Store vial at -20oC to -80oC. When stored at the recommended temperature, this protein is stable for 12 months.
Please avoid freeze-thaw cycles.
Purity
Greater than 90% as determined by SDS-PAGE.
Applications
? Protein Assay.
? Antibody Production.
? Western Blotting.
References
Title:Reduced Monomeric CD4 Is the Preferred Receptor for HIV.
Publication: 10.1074/jbc.M110.190579 December 24, 2010 The Journal of Biological Chemistry, 285, 40793-40799.
Link:http://www.jbc.org/content/285/52/40793.full#fn-1
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
