Catalogue number
PRO-1961
Synonyms
Mannose-6-Phosphate Receptor (Cation Dependent), CD-MPR, MPR46, CD Man-6-P Receptor, MPR 46, 46-KDa Mannose 6-Phosphate Receptor, MPR-46, SMPR, Cation-Dependent Mannose-6-Phosphate Receptor, Mr 46,000 Man6PR, Small Mannose 6-Phosphate Receptor, 46 KDa Mannose 6-Phosphate Receptor, MPRD, M6PR.
Introduction
Mannose-6-Phosphate Receptor (M6PR) belongs to the P-type lectin family. P-type lectins play a vital role in lysosome function through the specific transport of mannose-6-phosphate-containing acid hydrolases from the Golgi complex to lysosomes. The M6PR protein functions as a homodimer and needs divalent cations for ligand binding. Lysosomal enzymes carrying phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the ensuing receptor-ligand complex is transferred to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex.
Description
M6PR Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 182 amino acids (27-185) and having a molecular mass of 20.3kDa.
M6PR is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
The M6PR solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSTEEKTCD LVGEKGKESE KELALVKRLK PLFNKSFEST VGQGSDTYIY IFRVCREAGN HTSGAGLVQI NKSNGKETVV GRLNETHIFN GSNWIMLIYK GGDEYDNHCG KEQRRAVVMI SCNRHTLADN FNPVSEERGK VQDCFYLFEM DSSLACSPEI SH.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
