Catalogue number
PRO-1825
Synonyms
Cerebral Cavernous Malformation 2, C7orf22, malcavernin, Cerebral
Cavernous Malformations 2 Protein, Chromosome 7 Open Reading Frame 22, OSM, MGC4067.
Introduction
Cerebral
Cavernous Malformation 2, also known as CCM2 is a piece of the CCM signaling
pathway which is a vital regulator of heart and vessel formation as well as
integrity. CCM2 performs through the stabilization of endothelial cell
junctions. In addition, CCM2 functions as a scaffold protein for MAP2K3-MAP3K3
signaling. CCM2 plays a key role in the modulation of MAP3K3-dependent p38
activation induced by hyperosmotic shock. Mutations in CCM2 result in cerebral
cavernous malformations. Multiple transcript variants encoding dissimilar
isoforms have been discovered
for CCM2.
Description
CCM2 Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 311 amino acids (66-353 a.a) and
having a molecular mass of 34.3kDa.CCM2 is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
CCM2 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 20% glycerol and
1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze thaw cycles.
Purity
Greater
than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSEVKYLGQ LTSIPGYLNP SSRTEILHFI DNAKRAHQLP GHLTQEHDAV
LSLSAYNVKL AWRDGEDIIL RVPIHDIAAV SYVRDDAAHL VVLKTDDSST KVDIKETYEV EASTFCFPES
VDVGGASPHS KTISESELSA SATELLQDYM LTLRTKLSSQ EIQQFAALLH EYRNGASIHE FCINLRQLYG DSRKFLLLGL RPFIPEKDSQ
HFENFLETIG VKDGRGIITD SFGRHRRALS TTSSSTTNGN RATGSSDDRS APSEGDEWDR MISDISSDIE
ALGCSMDQDS A
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
