Catalogue number
PRO-1182
Synonyms
Nucleophosmin/nucleoplasmin 2, nucleoplasmin-2.
Introduction
NPM2 is a member of nucleoplasmin family. NPM2 is a core histones chaperone which takes part in chromatin reprogramming, particularly throughout fertilization and early embryonic development. NPM2 also takes part in sperm DNA decondensation during fertilization.
Description
NPM2 Human Recombinant produced in E. coli is a single polypeptide chain containing 237 amino acids (1-214) and having a molecular mass of 26.6 kDa.
NPM2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
E.coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
The NPM2 solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 200mM NaCl, 2mM DTT and 20% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMNLSSAS STEEKAVTTV LWGCELSQER RTWTFRPQLE GKQSCRLLLH TICLGEKAKE EMHRVEILPP ANQEDKKMQP VTIASLQASV LPMVSMVGVQ LSPPVTFQLR AGSGPVFLSG QERYEASDLT WEEEEEEEGE EEEEEEEDDE DEDADISLEE
QSPVKQVKRL VPQKQASVAK KKKLEKEEEE IRASVRDKSP VKKAKATARA KKPGFKK
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
