Catalogue number
ENZ-716
Synonyms
Asparagine--tRNA ligase, cytoplasmic, EC 6.1.1.22, Asparaginyl-tRNA synthetase, AsnRS, NARS, NARS1.
Introduction
Aminoacyl-tRNA synthetases are a class of enzymes which charge tRNAs with their cognate amino acids. Asparaginyl-tRNA synthetase (NARS) is localized to the cytoplasm and is a member of the class II family of tRNA synthetases. The N-terminal domain characterizes the signature sequence for the eukaryotic asparaginyl-tRNA synthetases.
Description
NARS Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 63,853 Dalton.
NARS is expressed with a -6xHis tag at N-terminus and purified by proprietary chromatographic techniques.
Source
Sf9 Insect Cells.
Physical Appearance
Sterile Filtered clear solution.
Formulation
NARS is supplied in 20mM HEPES buffer pH-7.6, 250mM NaCl and 20% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Purity
Greater than 80.0% as determined by SDS-PAGE.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
