Catalogue number
PKA-060
Synonyms
Thermosensitive gluconokinase, Gluconate kinase 1, idnK.
Introduction
Thermosensitive Gluconokinase, also known as IDNK is a 187 a.a protein which
is a member of the gluconokinase gntK/gntV family. IDNK catalyzes theconversion of ATP and D-gluconate to ADP and 6-phospho-D-gluconate. In
addition, IDNK is considered to take part in gender determination, deletion of
the distal portion of 9p is able to lead to a development of male to female sex
reversal, the phenotype of a female with a male X, Y genotype.
Description
IDNK E.Coli Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 210 amino acids (1-187 a.a) and
having a molecular mass of 23.4kDa.
IDNK is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
E.coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
IDNK protein solution (1mg/ml) containing 20mM Tris-HCl
buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 95% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMAGESFI LMGVSGSGKT LIGSKVAALL SAKFIDGDDL HPAKNIDKMS
QGIPLSDEDR LPWLERLNDA SYSLYKKNET GFIVCSSLKK QYRDILRKGS PHVHFLWLDG DYETILARMQ
RRAGHFMPVA LLKSQFEALE RPQADEQDIV RIDINHDIAN
VTEQCRQAVL AIRQNRICAK EGSASDQRCE.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
