Catalogue number
ENZ-863
Synonyms
Procollagen
C-Endopeptidase Enhancer, Procollagen C-Proteinase Enhancer 1, Type I
Procollagen COOH-Terminal Proteinase Enhancer, Type 1 Procollagen C-Proteinase
Enhancer Protein, Procollagen COOH-Terminal Proteinase Enhancer 1, PCPE-1, PCPE1,
Procollagen, Type 1, COOH-Terminal Proteinase Enhancer, Procollagen
C-Endopeptidase Enhancer 1, COOH-Terminal Proteinase Enhancer, Procollagen,
Type 1, PCPE, Procollagen C-endopeptidase enhancer 1.
Introduction
Procollagen C-endopeptidase enhancer 1, also known as PCOLCE binds to
the C-terminal propeptide of type I procollagen and enhances procollagen
C-proteinase activity. In addition, C-terminal processed part of PCPE (CT-PCPE)
has a metalloproteinase inhibitory activity. Among the diseases which are associated
with PCOLCE: bone fracture & oculopharyngeal muscular dystrophy.
Description
PCOLCE Human Recombinant produced in E.Coli is a single, non-glycosylated
polypeptide chain containing 447 amino acids (26-449 a.a) and having a
molecular mass of 47.9kDa.
PCOLCE is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile filtered colorless solution.
Formulation
PCOLCE protein solution (1mg/ml) containing 20mM Tris-HCl
buffer (pH 8.0) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH
SSGLVPRGSH MGSQTPNYTR
PVFLCGGDVK GESGYVASEG FPNLYPPNKE CIWTITVPEG QTVSLSFRVF DLELHPACRY DALEVFAGSG
TSGQRLGRFC GTFRPAPLVA PGNQVTLRMT TDEGTGGRGF LLWYSGRATS GTEHQFCGGR LEKAQGTLTT
PNWPESDYPP GISCSWHIIA PPDQVIALTF EKFDLEPDTY CRYDSVSVFN GAVSDDSRRL GKFCGDAVPG
SISSEGNELL VQFVSDLSVT ADGFSASYKT LPRGTAKEGQ GPGPKRGTEP KVKLPPKSQP PEKTEESPSA
PDAPTCPKQC RRTGTLQSNF CASSLVVTAT VKSMVREPGE GLAVTVSLIG AYKTGGLDLP SPPTGASLKF
YVPCKQCPPM KKGVSYLLMG QVEENRGPVL PPESFVVLHR PNQDQILTNL SKRKCPSQPV RAAASQD.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
