Catalogue number
ENZ-377
Synonyms
Cathepsin L, CTSL1, Cathepsin L1, Major Excreted Protein,
MEP, EC 3.4.22.15, CATL, EC 3.4.22.
Introduction
Cathepsin-L also known as CTSL is a member of the peptidase C1 family.
CTSL, is a dimer composed of disulfide-linked heavy and light chains, both
formed from a single protein precursor. Furthermore, CTSL is a lysosomal
cysteine proteinase which takes a main part in intracellular protein
catabolism. CTSL substrates include collagen and elastin, as well as alpha-1
protease inhibitor, which is the most important controlling element of
neutrophil elastase activity. CTSL has been implicated in a number of pathologic
processes, including myofibril necrosis in myopathies and in myocardial
ischemia, and in the renal tubular response to proteinuria. Multiple
alternatively spliced transcript variants have been found for CTSL.
Description
CTSL Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 339 amino acids (18-333 a.a) and
having a molecular mass of 38.3kDa.
CTSL is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
CTSL protein solution (1mg/ml) containing 20mM Tris-HCl (pH 8.0) and?10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze thaw cycles.
Purity
Greater
than 90% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSTLTFDHS LEAQWTKWKA MHNRLYGMNE EGWRRAVWEK NMKMIELHNQ EYREGKHSFT MAMNAFGDMT SEEFRQVMNG FQNRKPRKGK VFQEPLFYEA PRSVDWREKG YVTPVKNQGQ CGSCWAFSAT GALEGQMFRK TGRLISLSEQ NLVDCSGPQG NEGCNGGLMD YAFQYVQDNG GLDSEESYPY EATEESCKYN PKYSVANDTG FVDIPKQEKA LMKAVATVGP ISVAIDAGHE SFLFYKEGIY FEPDCSSEDM DHGVLVVGYG FESTESDNNK YWLVKNSWGE EWGMGGYVKM AKDRRNHCGI ASAASYPTV.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
