Catalogue number
ANT-589
Synonyms
Far upstream element (FUSE) binding protein 1, FUSE-binding protein 1, DNA helicase V, FUBP, FBP, hDH V.
Type
Mouse Anti Human Monoclonal.
Introduction
FUBP1 is a ssDNA binding protein which stimulates expression of c-myc in undifferentiated cells and activates the far upstream element (FUSE) of c-myc. Regulation of FUSE by FUBP happens by single-strand binding of FUBP to the non-coding strand. FUBP1 performs as an ATP-dependent DNA helicase.
Clone
PAT14F5AT.
Physical Appearance
Sterile filtered colorless solution.
Immunogen
Anti-human FUBP1 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human FUBP1 protein 279-448 amino acids purified from E. coli.
Ig Subclass
Mouse IgG2b heavy chain and k light chain.
Purification Method
FUBP1 antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
Formulation
1mg/ml containing PBS, pH-7.4, 10% Glycerol and 0.02% Sodium Azide.
Storage Procedures
For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C.?
Prevent freeze thaw cycles.
Stability / Shelf Life
12 months at -20°C. 1 month at 4°C.
Applications
FUBP1 antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution for Western blot analysis is 1:1000.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
