Catalogue number
ANT-562
Synonyms
ANX1,
LPC1, ANXA1, Lipocortin I, Calpactin II, Chrombindin-9, p35, Annexin-1,
Phospholipase A2 inhibitory protein, Annexin I, Annexin A1.
Type
Mouse Anti Human Monoclonal.
Introduction
ANXA1 is part of the family of Ca(2+)-dependent phospholipid binding proteins which have a Mw between 35kDa-40kDa and are situated on the cytosolic face of the plasma membrane. ANXA1 protein has a Mw of 40kDa, with phospholipase A2 inhibitory activity to bind from two to four calcium ions with high affinity. Since phospholipase A2 is necessary for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, ANXA1 might have potential anti-inflammatory activity. ANXA1 promotes membrane fusion and iplays a role in exocytosis. The recognition of ANXA1 protein by immunocytochemical leads a simple, highly sensitive and specific assay for diagnosis of hairy cell leukemia.
Clone
PAT2G5AT
Physical Appearance
Sterile filtered colorless solution.
Immunogen
Anti-human ANXA1 mAb,
clone PAT2G5AT, is derived from hybridization of mouse F0 myeloma cells with
spleen cells from BALB/c mice immunized with a recombinant human ANXA1 protein 1-346
amino acids purified from E. coli.
Ig Subclass
Mouse IgG1 heavy chain and k light chain.
Purification Method
ANXA1 antibody was purified from mouse ascitic fluids by
protein-A affinity chromatography.
Formulation
1mg/ml containing PBS, pH-7.4, 10% Glycerol and 0.02% Sodium Azide.
Storage Procedures
For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C.?
Prevent freeze thaw cycles.
Stability / Shelf Life
12 months at -20°C. 1 month at 4°C.
Applications
The antibody has been tested by ELISA, Western blot
analysis to assure specificity and reactivity. Since application varies,
however, each investigation should be titrated by the reagent to obtain optimal
results. Recommended starting dilution is 1:3000-1:20000.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
