Catalogue number
ANT-502
Synonyms
Protein phosphatase 1F, Ca(2+)/calmodulin-dependent
protein kinase phosphatase, CaM-kinase phosphatase, CaMKPase, Partner of PIX 2,
Protein fem-2 homolog, hFem-2, KIAA0015, POPX2, PPM1F, CAMKP, FEM-2.
Type
Mouse Anti Human Monoclonal.
Introduction
Protein Phosphatase 1F (PPM1F) is a part of the PP2C family of Ser/Thr protein phosphatases whose members are known to be negative regulators of cell stress response pathways. PPM1F interacts with Rho guanine nucleotide exchange factors (PIX), and as a result blocks the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is one of the substrates of PPM1F. PPM1F is found to be an apoptosis Promoter.
Clone
PAT6D11AT.
Physical Appearance
Sterile filtered colorless solution.
Immunogen
Anti-human PPM1F mAb,
clone PAT6D11AT, is derived from hybridization of mouse F0 myeloma cells with
spleen cells from BALB/c mice immunized with a recombinant human PPM1F protein 1-454
amino acids purified from E. coli.
Ig Subclass
Mouse IgG1 heavy chain and k light chain.
Purification Method
PPM1F antibody was purified from mouse ascitic fluids by
protein-A affinity chromatography.
Formulation
1mg/ml containing PBS, pH-7.4, 10% Glycerol and 0.02% Sodium Azide.
Storage Procedures
For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C.?
Prevent freeze thaw cycles.
Stability / Shelf Life
12 months at -20°C. 1 month at 4°C.
Applications
The
antibody has been tested by ELISA, Western blot analysis to assure specificity
and reactivity. Since application varies, however, each investigation should be
titrated by the reagent to obtain optimal results. Recommended starting
dilution is 1:1000.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
