Catalogue number
PRO-410
Synonyms
Calsequestrin-2, Calsequestrin cardiac muscle isoform, CASQ2, CSQ.
Description
Calsequestrin is the major calcium storage protein of the sarcoplasmic reticulum. Intraluminar Ca2+ binds to calsequestrin during diastole to prevent Ca2+ precipitation and to lower its free ionic concentration to facilitate efficient storage. During systole, Calsequestrin coordinately releases ~40--50 Ca2+ ions per molecule for each contraction-relaxation cycle by an uncertain mechanism. Calsequestrin has been shown to be of major importance in the regulation of cardiac excitation-contraction coupling.
Source
Dog Heart.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The protein was lyophilized from a concentrated solution (1mg/ml) containing 10mM Tris-HCl and 1mM EGTA.
Solubility
It is recommended to reconstitute the lyophilized CASQ2 in sterile 18M-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
Stability
Lyophilized CASQ2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CASQ2 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity
Greater than 90% as determined by SDS-PAGE.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
