Catalogue number
PRO-2123
Synonyms
Succinate Dehydrogenase Complex Assembly Factor 1, LYR
Motif-Containing Protein 8, LYR Motif
Containing 8, SDH Assembly Factor 1, LYRM8, Succinate Dehydrogenase Assembly
Factor 1, Mitochondrial, Succinate dehydrogenase assembly factor 1,
mitochondrial.
Introduction
Succinate dehydrogenase assembly factor 1 (SDHAF1) is a member of the complex
I LYR family. SDHAF1 plays an important role in succinate dehydrogenase complex
(SDH) assembly, a complex which is involved in complex II of the mitochondrial
electron transport chain. SDHAF1 functions through taking part in mitochondrial
biosynthesis of iron-sulfur centers for complex II.
Description
SDHAF1 Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 138 amino acids (1-115 a.a) and
having a molecular mass of 15.2kDa.
SDHAF1 is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
SDHAF1 protein solution (0.25mg/ml) containing Phosphate
buffered saline (pH7.4), 30% glycerol, 2mM DTT and 0.1mM PMSF.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMSRHSRL QRQVLSLYRD LLRAGRGKPG AEARVRAEFR QHAGLPRSDV
LRIEYLYRRG RRQLQLLRSG HATAMGAFVR PRAPTGEPGG VGSQPDDGDS PRNPHDSTGA PETRPDGR.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
