Catalogue number
PRO-1901
Synonyms
E3 ubiquitin-protein ligase RNF114, RING finger protein 114, Zinc finger protein 228, Zinc finger protein 313, RNF114, ZNF228, ZNF313, PSORS12.
Introduction
Ring Finger Protein 114 (RNF114) is expressed in various tissues, including skin, CD4 lymphocytes and dendritic cells. RNF114, which contains 1 RING-type zinc finger, takes part in spermatogenesis.
Description
RNF114 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 251 amino acids (1-228 a.a) and having a molecular mass of 28.1kDa.
RNF114 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
RNF114 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.4M UREA and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMAAQQRD CGGAAQLAGP AAEADPLGRF TCPVCLEVYE KPVQVPCGHV FCSACLQECL KPKKPVCGVC RSALAPGVRA VELERQIEST ETSCHGCRKN FFLSKIRSHV ATCSKYQNYI MEGVKATIKD ASLQPRNVPN RYTFPCPYCP EKNFDQEGLV EHCKLFHSTD TKSVVCPICA SMPWGDPNYR SANFREHIQR RHRFSYDTFV DYDVDEEDMM NQVLQRSIID Q.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
