Catalogue number
PRO-1753
Synonyms
NOP17, Nucleolar
Protein 17 Homolog, PIH1 Domain-Containing
Protein 1, PIH1 Domain-Containing 1, PIH1D1.
Introduction
PIH1 domain-containing protein 1 (PIH1D1) is a member of the PIH1
family. PIH1D1 is a phylogenetically conserved protein vital for efficient
processing of pre-rRNA through its association along with a class of small
nucleolar RNAs (snoRNAs) during ribosomal biogenesis. SnoRNAs are linked in
ribonucleoprotein particles localized to the nucleolus. PIH1D1 may also act as
a novel modulator of apoptosis pathway.
Description
PIH1D1 Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 313 amino acids (1-290a.a) and
having a molecular mass of 34.8kDa. PIH1D1 is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
PIH1D1 protein solution (0.25mg/ml) containing 20mM
Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze thaw cycles.
Purity
Greater
than 85% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMANPKLL GMGLSEAEAI GADSARFEEL LLQASKELQQ AQTTRPESTQ
IQPQPGFCIK TNSSEGKVFI NICHSPSIPP PADVTEEELL QMLEEDQAGF RIPMSLGEPH AELDAKGQGC
TAYDVAVNSD FYRRMQNSDF LRELVITIAR EGLEDKYNLQ LNPEWRMMKN RPFMGSISQQ NIRSEQRPRI QELGDLYTPA PGRAESGPEK
PHLNLWLEAP DLLLAEVDLP KLDGALGLSL EIGENRLVMG GPQQLYHLDA YIPLQINSHE SKAAFHRKRK
QLMVAMPLLP VPS
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
