Catalogue number
PRO-1891
Synonyms
Suppressor Of Cytokine Signaling 3, CIS3, SSI3, Cytokine-Inducible
SH2 Protein 3, STAT-Induced STAT Inhibitor 3, ATOD4, Cish3, MGC71791, CIS-3.
Introduction
Suppressor of Cytokine Signaling 3, also known as SOCS3 belongs to the SOCS family; in addition SOCS3 is a member of the STAT-induced STAT inhibitor (SSI). The SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of SOCS3 is induced by different cytokines, including IL6 and IL10. SOCS3 can bind to JAK2 kinase, and inhibit the activity of JAK2 kinase. Studies of the mouse counterpart of SOCS3 have shown the roles of SOCS3 in the negative regulation of fetal liver hematopoiesis, and placental development.
Description
SOCS3 Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 262 amino acids (1-225 a.a) and
having a molecular mass of 29kDa.
SOCS3 is fused to a 37 amino acid His-tag at
N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
SOCS3 protein solution (0.5mg/ml) containing 20mM Tris-HCl
buffer (pH 8.0), 0.4M Urea and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze thaw cycles.
Purity
Greater
than 90% as determined by SDS-PAGE.
Amino acid sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMVT HSKFPAAGMS RPLDTSLRLK TFSSKSEYQL VVNAVRKLQE
SGFYWSAVTG GEANLLLSAE PAGTFLIRDS SDQRHFFTLS VKTQSGTKNL RIQCEGGSFS LQSDPRSTQP
VPRFDCVLKL VHHYMPPPGA PSFPSPPTEP SSEVPEQPSA QPLPGSPPRR AYYIYSGGEK IPLVLSRPLS
SNVATLQHLC RKTVNGHLDS YEKVTQLPGP IREFLDQYDA PL
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
