Catalogue number
HOR-304
Synonyms
Procalcitonin, PCT.
Introduction
Procalcitonin is a peptide hormone mainly produced by the C cells of the thyroid and certain endocrine cells of the lung. Under normal expression conditions, procalcitonin is immediately cleaved into three specific fragments, an N terminal residue, calcitonin and katacalcin. Levels of unprocessed procalcitonin rise significantly after bacterial infection, trauma or shock.
Description
Procalcitonin Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 116 amino acids and having a molecular mass of 12.8 kDa.
The Procalcitonin is purified by standard chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from 10mM sodium phosphate pH-7.5.
Solubility
It is recommended to reconstitute the lyophilized procalcitonin sterile 18MΩ-cm H2O at 100 μg/ml, which can then be further diluted to other aqueous solutions.
Stability
Lyophilized procalcitonin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution procalcitonin should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Amino acid sequence
APFRSALESS PADPATLSED EARLLLAALV QDYVQMKASE LEQEQEREGS SLDSPRSKRC GNLSTCMLGT YTQDFNKFHT FPQTAIGVGA PGKKRDMSSD LERDHRPHVS MPQNAN.
Biological Activity
Human Procalcitonin was tested using Biomerieux reagents on the Min-Vidas.
The assay was run at 80ng/ml with a typical recovery of 80 percent.
References
Title:A sandwich assay for procalcitonin detection in POCT applications.
Publication:Advanced Biomedical and Clinical Diagnostic Systems VII, edited by Anita Mahadevan-Jansen,Tuan Vo-Dinh, Warren S. Grundfest, Proc. of SPIE Vol. 7169, 716905? 2009 SPIE · CCC code: 1605-7422/09/$18 · doi: 10.1117/12.810265
Link:http://144.206.159.178/ft/CONF/16425379/16425383.pdf
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
