Catalogue number
CYT-401
Synonyms
Macrophage Migration Inhibitory Factor
(Glycosylation-Inhibiting Factor), Phenylpyruvate Tautomerase, L-Dopachrome
Tautomerase, L-Dopachrome Isomerase,
GLIF, MMIF, GIF, Macrophage
Migration Inhibitory Factor, Glycosylation-Inhibiting Factor, EC 5.3.3.12, EC
5.3.2.1, Macrophage migration inhibitory factor.
Introduction
The cytokine Macrophage migration inhibitory factor (MIF) has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration.
Description
MIF Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 345 amino acids (1-115 a.a) and
having a molecular mass of 39.2kDa.
MIF is fused to a 230 amino
acid GST-tag at N-terminus & purified by proprietary chromatographic
techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
MIF protein solution (1mg/ml) containing Phosphate
Buffered Saline (pH7.4) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MSPILGYWKI
KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN
MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH
PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD
LVPRGSPEFA
MPMFIVNTNV PRASVPDGFL SELTQQLAQA TGKPPQYIAV HVVPDQLMAF GGSSEPCALC SLHSIGKIGG
AQNRSYSKLL CGLLAERLRI SPDRVYINYY DMNAANVGWN NSTFA.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
