Catalogue number
PRO-1398
Synonyms
GULP, Engulfment Adaptor PTB Domain Containing 1, PTB
Domain Adapter Protein CED-6, Cell Death Protein 6 Homolog, CED6, GULP, PTB Domain Adaptor Protein
CED-6 Engulfment Adapter Protein,
Protein GULP, CED-6.
Introduction
PTB domain-containing engulfment adapter protein 1 (GULP1) is an evolutionarily conserved adaptor protein essential for efficient phagocytosis of apoptotic cells by phagocytes. Furthermore, GULP1 helps modulate cellular glycosphingolipid and cholesterol transport. In addition, GULP1 may have a role in the internalization and endosomal trafficking of various LRP1 ligands, such as PSAP. Increased cytoplasmic levels of GULP1 are linked with increases in cellular levels of GTP-bound ARF6.
Description
GULP1 Human Recombinant produced in E. coli is a single,
non-glycosylated polypeptide chain containing 327 amino acids (1-304a.a) and
having a molecular mass of 36.9kDa.
GULP1 is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile
filtered colorless solution.
Formulation
GULP1 protein solution (0.5mg/ml) containing 20mM
Tris-HCl buffer (pH 8.0), 0.15M NaCl, 50% glycerol and 2mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater
than 95.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMNRAFSR KKDKTWMHTP EALSKHFIPY NAKFLGSTEV EQPKGTEVVR
DAVRKLKFAR HIKKSEGQKI PKVELQISIY GVKILEPKTK EVQHNCQLHR ISFCADDKTD KRIFTFICKD
SESNKHLCYV FDSEKCAEEI TLTIGQAFDL AYRKFLESGG KDVETRKQIA GLQKRIQDLE TENMELKNKV
QDLENQLRIT QVSAPPAGSM TPKSPSTDIF DMIPFSPISH QSSMPTRNGT QPPPVPSRST EIKRDLFGAE
PFDPFNCGAA DFPPDIQSKL DEMQEGFKMG LTLEGTVFCL DPLDSRC.
Safety Data Sheet
Usage
ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
