Catalogue number
PRO-302
Synonyms
BolA-like protein 1, hBolA, BOLA1, CGI-143.
Introduction
BolA Homolog 1(BOLA1) is a member of the bolA/yrbA family. BolA proteins, which include the: hBolA1, hBolA2, and hBolA3, are novel non-classical secreted proteins detected with bioinformatics and molecular biology experiments. The 3 BolA fusion proteins with c-Myc tag can be secreted into the culture medium of the transfected Cos-7 cells, even though they could not be colocalized with Golgi apparatus. Also, the secretion of 3 BolA proteins could not be inhibited after BFA treatment.
Description
BOLA1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 140 amino acids (21-137 a.a) and having a molecular mass of 14kDa.
BOLA1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
BOLA1 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 20% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSQGSAGSG AIGPVEAAIR TKLEEALSPE VLELRNESGG HAVPPGSETH FRVAVVSSRF EGLSPLQRHR LVHAALAEEL GGPVHALAIQ ARTPAQWREN SQLDTSPPCL GGNKKTLGTP.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
