Catalogue number
PRO-2314
Synonyms
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Inositol monophosphatase
3, IMP 3, IMPase 3, Golgi 3-prime phosphoadenosine 5-prime phosphate 3-prime
phosphatase, Golgi-resident PAP phosphatase, gPAPP, Inositol monophosphatase
domain-containing protein 1, Inositol-1(or 4)-monophosphatase 3 Myo-inositol
monophosphatase A3, Impad1, Impa3. |
Introduction
Inositol monophosphatase 3 (IMPAD1) belongs to the inositol monophosphatase family. IMPAD1 is restricted to the Golgi apparatus and catalyzes the hydrolysis of phosphoadenosine phosphate (PAP) to adenosine monophosphate (AMP). IMPAD1 gene mutations cause the GRAPP type chondrodysplasia with joint dislocations, and a pseudogene of the IMPAD1 gene is located on the long arm of chromosome 1.
Description
IMPAD1 produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 332 amino acids (34-356aa) and having a molecular mass of 36.2kDa (Molecular size on SDS-PAGE will appear at approximately 28-40kDa).
IMPAD1 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Source
Sf9
Insect cells.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
IMPAD1 protein solution (0.5mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Amino acid sequence
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ADPGRFSLFG LGSEPAAGEA EVASDGGTVD LREMLAVAVL AAERGGDEVR
RVRESNVLHE KSKGKTREGA DDKMTSGDVL SNRKMFYLLK?TAFPNVQINT EEHVDASDKE
VIVWNRKIPE DILKEIAAPK EVPAESVTVW IDPLDATQEY TEDLRKYVTT MVCVAVNGKP VLGVIHKPFS?EYTAWAMVDG GSNVKARSSY
NEKTPKIIVS RSHAGMVKQV ALQTFGNQTS IIPAGGAGYK VLALLDVPDM TQEKADLYIH VTYIKKWDIC AGNAILKALG GHMTTLNGEE
ISYTGSDGIE GGLLASIRMN HQALVRKLPD LEKSGHHHHH HH. |
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
