Catalogue number
PRO-1993
Synonyms
Exosome component 1, CGI-108, CSL4, Csl4p, hCsl4p, p13, RP11-452K12.9, SKI4, Ski4p, Exosome complex component CSL4, Exosome component 1, EXOSC1.
Introduction
EXOSC1 is a non-catalytic component of the exosome which has 3'->5' exoribonuclease activity and participates in many cellular RNA processing and degradation occasions. EXOSC1 associates with the exosome by protein-protein interactions with ribosomal RNA-processing protein 42 and ribosomal RNA-processing protein 46.
Description
EXOSC1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 218 amino acids (1-195a.a) and having a molecular mass of 23.8kDa.
EXOSC1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
EXOSC1 protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 20% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMAPPVRY CIPGERLCNL EEGSPGSGTY TRHGYIFSSL AGCLMKSSEN GALPVVSVVR ETESQLLPDV GAIVTCKVSS INSRFAKVHI LYVGSMPLKN SFRGTIRKED VRATEKDKVE IYKSFRPGDI VLAKVISLGD AQSNYLLTTA ENELGVVVAH SESGIQMVPI SWCEMQCPKT HTKEFRKVAR VQPEFLQT.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
