Catalogue number
PRO-654
Synonyms
GIP, CTRP1, ZSIG37, FLJ90694, C1QTNF1, Complement C1q Tumor Necrosis Factor-Related Protein 1, G protein-coupled receptor-interacting protein.
Introduction
C1QTNF1 is a novel adipokine, providing a significant framework to further address the physiological functions and mechanisms of the action of this family of secreted glycoproteins in normal and disease states. C1QTNF1 increases the production of aldosterone. C1QTNF1 is vastly expressed in obese subjects as well as up-regulated in hypertensive patients, C1QTNF1 is identified molecular link between obesity and hypertension. C1QTNF1 expression may be associated with a low-grade chronic inflammation status in adipose tissues.
Description
C1QTNF1 Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 266 amino acids and having a molecular mass of 30.45 kDa. The protein contains an extra His tag at N-terminus. The C1QTNF1 amino acid sequence is identical to UniProtKB/Swiss-Prot entry Q9BXJ1 amino acids 26–281.
The C1QTNF1 is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Formulation
Human C1QTNF1 was lyophilized from 50mM Acetate Buffer pH-4.
Solubility
Add 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5mg/mL and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/ml. In higher concentrations the solubility of this antigen is limited. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Stability
Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Purity
Greater than 95% as determined by SDS PAGE.
Amino acid sequence
MKHHHHHHAS?RVPHVQGEQQ EWEGTEELPS PPDHAERAEE QHEKYRPSQD QGLPASRCLR CCDPGTSMYP ATAVPQINIT ILKGEKGDRG DRGLQGKYGK TGSAGARGHT GPKGQKGSMG APGERCKSHY AAFSVGRKKP MHSNHYYQTV IFDTEFVNLY DHFNMFTGKF YCYVPGLYFF SLNVHTWNQK ETYLHIMKNE EEVVILFAQV GDRSIMQSQS LMLELREQDQ VWVRLYKGER ENAIFSEELD TYITFSGYLVKHATEP.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
