Caused by one of four closely related virus serotypes of the genus Flavivirus, family Flaviviridae, each serotype is sufficiently different that there is no cross-protection and epidemics caused by multiple serotypes (hyperendemicity) can occur. In cell culture experiments and mice Morpholino antisense oligos have shown specific activity against Dengue virus.
Recombinant Dengue p18 antigen is a genetically engineered protein generated from dengue envelope protein, containing most dengue subtype epitopes. Specifically developed for dengue IgG/IgM lateral flow immunoassay. Recombinant Dengue p18 can detect dengue IgG and IgM from all four infected dengue virus subtypes. Having over 90% sensitivity and specify to detect dengue IgM and IgG. This antigen gives a good performance to detect dengue IgM with strong signal intensity.
Escherichia Coli.
Protein is >95% pure as determined by 12% PAGE (coomassie staining).
Phosphate buffered saline, pH 7.4 and 0.02% sodium nitrate.
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Protocol to prepare dengue IgG/IgM rapid test:
1. Prepare 532 to 534nm colloid gold, conjugation within 24 hours after colloid gold preparation;?
2. Adjust colloid gold OD at 1.3 to 1.4, conjugation concentration and pH are 16 to 18 ug/ml gold and 7.2;
3. Shanking conjugation for 6-8 hours, then centrifuge the conjugation at 10,000 rpm at 18oC for 20 min;
4. Remove the supernatnant as more as possible, add the?supplied gold suspension buffer;
5. Add the?supplied blocking reagent?to the final concentration to 0.4%;
6. Blocking for 1 hours , then add sucrose to the final concentration to 20%;
7. Dipping the conjugated gold to the gold pad ( do not use sodium phosphate for gold pad treatment);
8. After drying, assemble gold pad, the?supplied sample pad?and absorbent pad over backing card;
9. Add 10ul serum/plasma and add two drops running buffer for assay.
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
