Catalogue number
ENZ-929
Synonyms
NTMT1, N-Terminal Xaa-Pro-Lys N-Methyltransferase 1, X-Pro-Lys
N-Terminal Protein Methyltransferase 1A, Alpha
N-Terminal Protein Methyltransferase 1A , Methyltransferase-Like Protein 11A , N-Terminal RCC1 Methyltransferase, METTL11A, C9orf32, NTM1A, NRMT, Chromosome 9 Open
Reading Frame 32, Methyltransferase Like 11A, EC 2.1.1.244, AD-003, HOMT1A, NRMT1.
Introduction
N-terminal Xaa-Pro-Lys N-methyltrasferase1, also known as NTMT1 belongs
to the methyltransferase superfamily. NTMT1 catalyzes the
transfer of the methyl group from the S-adenosyl-l-methionine to the protein
?-amine, resulting in the formation of S-adenosyl-l-homocysteine and ?-N-methylated proteins. NTMT1 is a remarkable
potential anticancer target since it is
overexpressed in gastrointestinal cancers in addition to his essential function
in cell mitosis.
Description
NTMT1 Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 248 amino acids (1-223 a.a) and
having a molecular mass of 28.1kDa. NTMT1 is fused to a 25 amino acid His-tag
at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
NTMT1
protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M
NaCl and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater
than 95.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSEFMTSEV IEDEKQFYSK AKTYWKQIPP TVDGMLGGYG HISSIDINSS RKFLQRFLRE GPNKTGTSCA LDCGAGIGRI TKRLLLPLFR EVDMVDITED FLVQAKTYLG EEGKRVRNYF CCGLQDFTPE PDSYDVIWIQ WVIGHLTDQH LAEFLRRCKG
SLRPNGIIVI KDNMAQEGVI LDDVDSSVCR DLDVVRRIIC SAGLSLLAEE RQENLPDEIY HVYSFALR.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
