Catalogue number
ENZ-919
Synonyms
Acyl-CoA Synthetase Family Member 2, PPARG Binding, Long
Chain Fatty Acid Acyl Co-A Ligase Like, Acyl-CoA Synthetase Family Member 2,
Mitochondrial, EC 6.2.1.26, EC 6.2.1.-, FLJ20920, EC 6.2.1, AVYV493, ACSMW, Acyl-CoA
synthetase family member 2, mitochondrial.
Introduction
Acyl-CoA synthetase family member 2, also known as ACSF2 is a member of
the ATP-dependent AMP-binding enzyme family. Acyl-CoA synthetases are a family
of enzymes which catalyze the thioesterification of fatty acids with
coenzymeA to form activated intermediates, which play a basic part in lipid
metabolism as well as homeostasis of lipid-related processes. ACSF2 is required
for the complex of lipid synthesis, energy production via beta-oxidation,
protein acylation and fatty-acid dependent transcriptional regulation.
Moreover, ACSF2 is required for fatty acid import into cells by the process of
vectorial acylation.
Description
ACSF2 Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 597 amino acids (42-615 a.a) and
having a molecular mass of 66.1kDa.
ACSF2 is fused to a 23
amino acid His-tag at N-terminus & purified by proprietary chromatographic
techniques.
Source
E.coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
ACSF2 protein solution (0.5mg/ml) containing Phosphate
Buffered Saline pH 7.4 and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 95% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSLSSREVD RMVSTPIGGL SYVQGCTKKH LNSKTVGQCL ETTAQRVPER
EALVVLHEDV RLTFAQLKEE VDKAASGLLS IGLCKGDRLG MWGPNSYAWV LMQLATAQAG IILVSVNPAY
QAMELEYVLK KVGCKALVFP KQFKTQQYYN VLKQICPEVE NAQPGALKSQ RLPDLTTVIS VDAPLPGTLL
LDEVVAAGST RQHLDQLQYN QQFLSCHDPI NIQFTSGTTG SPKGATLSHY NIVNNSNILG ERLKLHEKTP
EQLRMILPNP LYHCLGSVAG
TMMCLMYGAT LILASPIFNG KKALEAISRE RGTFLYGTPT MFVDILNQPD
FSSYDISTMC GGVIAGSPAP PELIRAIINK INMKDLVVAY GTTENSPVTF AHFPEDTVEQ KAESVGRIMP
HTEARIMNME AGTLAKLNTP GELCIRGYCV MLGYWGEPQK TEEAVDQDKW YWTGDVATMN EQGFCKIVGR
SKDMIIRGGE NIYPAELEDF FHTHPKVQEV QVVGVKDDRM GEEICACIRL KDGEETTVEE IKAFCKGKIS
HFKIPKYIVF VTNYPLTISG KIQKFKLREQ MERHLNL.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
