Catalogue number
PKA-038
Synonyms
Glycerol
kinase, glycerol 3-phosphotransferase, Glycerokinase, GK.
Introduction
GlpK
also known as glycerol kinase, is a member of the FGGY kinase family. GlpK
catalyzes the transfer of a phosphate group from ATP to glycerol, thereby
forming glycerol phosphate. Furthermore, this intermediate can then be
converted to dihydroxyacetone phosphate (DHAP), which is utilized in either
glycolysis or gluconeogenesis. The activity of GlpK is affected by numerous metabolites.
The non-competitive allosteric inhibition by fructose 1,6-bisphosphate (FBP) triggers
modifications in the quaternary structure of Glpk.
Description
GlpK E. Coli Recombinant produced in E. coli is a single, non-glycosylated polypeptide chain containing 525 amino acids (1-502 a.a) and having a molecular mass of 58.6 kDa.
GlpK is fused to a 23 amino acid His-tag at N-terminus& purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
GlpK protein solution (1mg/ml) containing Phosphate
buffered saline (pH7.4),10% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater
than 95.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH
SSGLVPRGSH MGSMTEKKYI
VALDQGTTSS RAVVMDHDAN IISVSQREFE QIYPKPGWVE HDPMEIWATQ SSTLVEVLAK ADISSDQIAA
IGITNQRETT IVWEKETGKP IYNAIVWQCR RTAEICEHLK RDGLEDYIRS NTGLVIDPYF SGTKVKWILD
HVEGSRERAR RGELLFGTVD TWLIWKMTQG RVHVTDYTNA SRTMLFNIHT LDWDDKMLEV LDIPREMLPE
VRRSSEVYGQ TNIGGKGGTR IPISGIAGDQ QAALFGQLCV KEGMAKNTYG TGCFMLMNTG EKAVKSENGL LTTIACGPTG
EVNYALEGAV FMAGASIQWL RDEMKLINDA YDSEYFATKV QNTNGVYVVP AFTGLGAPYW DPYARGAIFG
LTRGVNANHI IRATLESIAY QTRDVLEAMQ ADSGIRLHAL RVDGGAVANN FLMQFQSDIL GTRVERPEVR
EVTALGAAYL AGLAVGFWQN LDELQEKAVI EREFRPGIET TERNYRYAGW KKAVKRAMAW EEHDE.
Safety Data Sheet
Usage
ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
