Catalogue number
ENZ-266
Synonyms
Serine/threonine-protein phosphatase 4 catalytic subunit,
PP4, PP4C, PPH3, PPP4, PPX, PPP4C, Protein Phosphatase 4 Catalytic subunit, Protein
phosphatase X, PP-X, Pp4.
Introduction
Protein Phosphatase 4 Catalytic subunit (PPP4C), is a part of the Serine/threonine-protein phosphatase catalytic subunits which tskes part in dephosphorylation and regulation of HDAC3. The protein phosphatase (PP) holoenzyme is a trimeric complex compound of a regulatory subunit, a variable subunit and a catalytic subunit. 4 major families of protein phosphatase catalytic subunits have been identified, designated PP1, PP2A, PP2B (calcineurin) and PP2C.
Description
PPP4C Human Recombinant produced in E. coli is a single polypeptide
chain containing 330 amino acids (1-307) and having a molecular mass of 37.5kDa.
PPP4C is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
E.coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
The PPP4C solution (1mg/1ml) contains 20mM Tris-HCl
buffer (pH 8.0), 0.4M Urea and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMAEISDL DRQIEQLRRC ELIKESEVKA LCAKAREILV EESNVQRVDS
PVTVCGDIHG QFYDLKELFR VGGDVPETNY LFMGDFVDRG FYSVETFLLL LALKVRYPDR ITLIRGNHES
RQITQVYGFY DECLRKYGSV TVWRYCTEIF DYLSLSAIID GKIFCVHGGL SPSIQTLDQI RTIDRKQEVP
HDGPMCDLLW SDPEDTTGWG VSPRGAGYLF GSDVVAQFNA ANDIDMICRA HQLVMEGYKW HFNETVLTVW
SAPNYCYRCG NVAAILELDE HLQKDFIIFE AAPQETRGIP SKKPVADYFL.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
