Catalogue number
ENZ-749
Synonyms
NADH Dehydrogenase (Ubiquinone) Flavoprotein 3 10kDa, Renal Carcinoma Antigen NY-REN-4, NADH-Ubiquinone Oxidoreductase 9 KDa Subunit, EC 1.6.5.311, CI-10k, Complex I 10kDa
Introduction
NDUFV3 is one of no less than 41 subunits which form the NADH-ubiquinone oxidoreductase complex that is involved in the mitochondrial respiratory chain and assists in catalyzing the rotenone-sensitive oxidation of NADH and the reduction of ubiquinone. NDUFV3 is 1 of 3 proteins found in the flavoprotein fraction of the complex but it exact function is unknown. Two transcript variants encoding altered isoforms are known for this gene.
Description
NDUFV3 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 97 amino acids (35-108) and having a molecular mass of 10.8kDa.
NDUFV3 is fused to a 23 amino acid His-
tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
The NDUFV3 solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 0.2M NaCl, 2mM DTT, 2mM EDTA and 50% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSSAESGKS EKGQPQNSKK QSPPKKPAPV PAEPFDNTTY KNLQHHDYST YTFLDLNLEL SKFRMPQPSS GRESPRH
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
