Catalogue number
DEN-033
Introduction
Caused by one of four closely related virus serotypes of the genus Flavivirus, family Flaviviridae, each serotype is sufficiently different that there is no cross-protection and epidemics caused by multiple serotypes (hyperendemicity) can occur. In cell culture experiments and mice Morpholino antisense oligos have shown specific activity against Dengue virus.
Description
Recombinant Dengue Virus Subtype 1 produced in Insect Cells is a polypeptide chain containing amino acids 281-675 and having a molecular weight of approximately 50kDa.
Dengue Envelope-1 is purified by proprietary chromatographic technique.
Source
Insect cells.
Physical Appearance
Sterile filtered colorless solution.
Formulation
Dengue Envelope-1 protein solution in 1xD-PBS, pH7.4, 0.1% Thimerosal, 5mM EDTA, 1μg/ml of Leupeptin, Aprotinin and Pepstatin A.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Protein is >95% pure as determined by 12.5% SDS-PAGE.
Amino acid sequence
mrcvgignrd fveglsgatw vdvvlehgsc vttmakdkpt ldiellktev tnpavlrklc ieakisnttt dsrcptqgea tlveeqdanf vcrrtfvdrg wgngcglfgk gslltcakfk cvtklegkiv qyenlkysvi vtvhtgdqhq vgnettehgt iatitpqapt seiqltdyga ltldcsprtg ldfnemvllt mkekswlvhk qwfldlplpw tsgastsqet wnrqdllvtf ktahakkqea vvlgsqegam htaltgatei qtsgtttifa ghlkcrlkmd kltlkgisyv mctgsfklek evaetqhgtv lvqvkyegtd apckipfssq dekgvtqngr lvtanpivtd kekpvnieae ppfgesyivv gagekalkls wfkkg.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
