Catalogue number
PKA-078
Synonyms
cAMP-dependent protein kinase type II-alpha regulatory
subunit, PKR2, PRKAR2.
Introduction
cAMP-dependent protein kinase type II-alpha regulatory subunit (PRKAR2A) can be phosphorylated by the activated catalytic subunit. PRKAR2A interacts with numerous A-kinase anchoring proteins and regulates the subcellular localization of cAMP-dependent protein kinase.
Description
PRKAR2A Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 434 amino acids (1-404 a.a) and having a molecular mass of 48.6kDa.
PRKAR2A is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
PRKAR2A protein solution (0.5mg/ml) in Phosphate buffered saline (pH7.4), 10% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMSHIQIP PGLTELLQGY TVEVLRQQPP DLVEFAVEYF TRLREARAPA
SVLPAATPRQ SLGHPPPEPG PDRVADAKGD SESEEDEDLE VPVPSRFNRR VSVCAETYNP DEEEEDTDPR
VIHPKTDEQR CRLQEACKDI LLFKNLDQEQ LSQVLDAMFE RIVKADEHVI DQGDDGDNFY VIERGTYDIL
VTKDNQTRSV GQYDNRGSFG ELALMYNTPR AATIVATSEG SLWGLDRVTF RRIIVKNNAK KRKMFESFIE
SVPLLKSLEV SERMKIVDVI GEKIYKDGER IITQGEKADS FYIIESGEVS ILIRSRTKSN KDGGNQEVEI
ARCHKGQYFG ELALVTNKPR AASAYAVGDV KCLVMDVQAF ERLLGPCMDI MKRNISHYEE QLVKMFGSSV
DLGNLGQ.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
