Catalogue number
PKA-054
Synonyms
MOB
Kinase Activator 1B, Mps One Binder Kinase Activator-Like 1A, Mob1 Homolog 1A, MOBKL1A,
Mob1A, MOB4A, MOB1, Mps One Binder Kinase Activator-Like 1A (Yeast), MOB1, Mps
One Binder Kinase Activator-Like 1A, MOB1 Mps One Binder Homolog B (Yeast), MOB1
Mps One Binder Homolog B, Mob4A Protein, MATS2, Mob1B, MOB kinase activator 1B.
Introduction
MOB Kinase Activator 1B (MOB1B) is similar to the yeast Mob1 protein. Yeast Mob1 binds Mps1p, which is a protein kinase vital for spindle pole body duplication and mitotic checkpoint regulation.
Description
MOB1B Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 239 amino acids (1-216 a.a) and
having a molecular mass of 27kDa.
MOB1B is fused to a 23
amino acid His-tag at N-terminus & purified by proprietary chromatographic
techniques.
Source
E.coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
MOB1B
protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl,
30% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 95% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH
SSGLVPRGSH MGSMSFLFGS
RSSKTFKPKK NIPEGSHQYE LLKHAEATLG SGNLRMAVML PEGEDLNEWV AVNTVDFFNQ INMLYGTITD
FCTEESCPVM SAGPKYEYHW ADGTNIKKPI KCSAPKYIDY LMTWVQDQLD DETLFPSKIG VPFPKNFMSV
AKTILKRLFR VYAHIYHQHF DPVIQLQEEA HLNTSFKHFI FFVQEFNLID RRELAPLQEL IEKLTSKDR.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
