Catalogue number
ENZ-972
Synonyms
Serpnie2, B230326M24Rik, PAI-1, PI-7, PI7, PN-1, Spi4, Glia-derived
nexin, GDN,Peptidase inhibitor 7, Protease nexin 1, Protease nexin I, Serine
protease-inhibitor 4, Serpin E2.
Introduction
Plasminogen
Activator Inhibitor-2 (Serpine2) which inhibits thrombin, plasmin and
plasminogen activators is a part of the Serpin superfamily of the serine
protease inhibitors. Serpine2 is able to transform human embryonic kidney cells
into neuron-like cells. Furthermore, Serpine2's over expression in mice leads
to progressive neuronal and motor dysfunction.
Description
SERPINE2 produced in Sf9 Baculovirus cells is a single,
glycosylated polypeptide chain containing 386 amino acids (20-397a.a.) and
having a molecular mass of 42.9kDa (Molecular size on SDS-PAGE will appear at
approximately 40-57kDa).
SERPINE2 is expressed with a 8 amino acid His tag at
C-Terminus and purified by proprietary chromatographic techniques.
Source
Sf9, Baculovirus cells.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
SERPINE2 protein solution (0.5mg/ml) contains Phosphate
Buffered Saline (pH 7.4) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater
than 95.0% as determined by SDS-PAGE.
Amino acid sequence
SQFNSLSLEE LGSNTGIQVF NQIIKSRPHE NVVVSPHGIA SILGMLQLGA
DGKTKKQLST VMRYNVNGVG KVLKKINKAI VSKKNKDIVT VANAVFLRNG FKMEVPFAVR NKDVFQCEVQ
NVNFQDPASA SESINFWVKN ETRGMIDNLL SPNLIDGALT RLVLVNAVYF KGLWKSRFQP ESTKKRTFVA
GDGKSYQVPM LAQLSVFRSG STRTPNGLWY NFIELPYHGE SISMLIALPT ESSTPLSAII PHITTKTIDS
WMNTMVPKRM QLVLPKFTAV AQTDLKEPLK ALGITEMFEP SKANFTKITR SESLHVSHIL QKAKIEVSED
GTKASAATTA ILIARSSPPW FIVDRPFLFS IRHNPTGAIL FLGQVNKPLE HHHHHH.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
