Catalogue number
ENZ-821
Synonyms
O-Sialoglycoprotein Endopeptidase, T(6)A37
Threonylcarbamoyladenosine Biosynthesis Protein OSGEP, TRNA
Threonylcarbamoyladenosine Biosynthesis Protein OSGEP, N6-L-Threonylcarbamoyladenine
Synthase, T(6)A Synthase, HOSGEP, GCPL1, Probable TRNA
Threonylcarbamoyladenosine Biosynthesis Protein OSGEP, Probable TRNA
N6-Adenosine Threonylcarbamoyltransferase , Probable O-Sialoglycoprotein
Endopeptidase, EC 3.4.24.57, EC 2.3.1.234, FLJ20411, OSGEP1, PRSMG1, KAE1, Probable
tRNA N6-adenosine threonylcarbamoyltransferase.
Introduction
O-Sialoglycoprotein
Endopeptidase, also known as OSGEP is a member of the KAE1 / TsaD family.? OSGEP is essential for the formation of
threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs which read
codons beginning with adenine. OSGEP take a direct catalytic part in the above
reaction, however other proteins of the complex are required to fulfill this
activity.
Description
OSGEP Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 358 amino acids (1-335 a.a) and
having a molecular mass of 38.8kDa.
OSGEP is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile filtered colorless solution.
Formulation
OSGEP protein solution (0.5mg/ml) containing 20mM
Tris-HCl buffer (pH8.0), 10% glycerol and 0.4M Urea.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 80.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMPAVLGF EGSANKIGVG VVRDGKVLAN PRRTYVTPPG TGFLPGDTAR
HHRAVILDLL QEALTESGLT SQDIDCIAYT KGPGMGAPLV SVAVVARTVA QLWNKPLVGV NHCIGHIEMG
RLITGATSPT VLYVSGGNTQ VIAYSEHRYR IFGETIDIAV
GNCLDRFARV LKISNDPSPG YNIEQMAKRG KKLVELPYTV KGMDVSFSGI
LSFIEDVAHR MLATGECTPE DLCFSLQETV FAMLVEITER AMAHCGSQEA LIVGGVGCNV RLQEMMATMC
QERGARLFAT DERFCIDNGA MIAQAGWEMF RAGHRTPLSD SGVTQRYRTD EVEVTWRD.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
