Catalogue number
ENZ-866
Synonyms
Monoamine Oxidase A, Monoamine Oxidase Type A, EC 1.4.3.4,
MAO-A, Amine Oxidase [Flavin-Containing] A, EC 1.4.3, Amine oxidase
[flavin-containing] A, Monoamine oxidase type A.
Introduction
Monoamine Oxidase A, also known as MAOA catalyzes the oxidative deamination of biogenic as well as xenobiotic amines and has significant functions in the metabolism of neuroactive and vasoactive amines in the central nervous system as well as peripheral tissues. Mutation in MAOA results in Brunner syndrome; in addition MAOA has also been linked with a diversity of other psychiatric disorders, which includes antisocial behavior. MAOA preferentially oxidizes biogenic amines such as 5-hydroxytryptamine (5-HT).
Description
MAOA Human Recombinant produced in E.Coli is a single, non-glycosylated
polypeptide chain containing 520 amino acids (1-497 a.a) and having a molecular
mass of 58.8kDa.?
MAOA is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
E.coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
MAOA
protein solution (0.5mg/ml) containing 20mM Tris-HCl (pH8.0) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 80% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMENQEKA SIAGHMFDVV VIGGGISGLS AAKLLTEYGV SVLVLEARDR
VGGRTYTIRN EHVDYVDVGG AYVGPTQNRI LRLSKELGIE TYKVNVSERL VQYVKGKTYP FRGAFPPVWN
PIAYLDYNNL WRTIDNMGKE IPTDAPWEAQ HADKWDKMTM KELIDKICWT KTARRFAYLF VNINVTSEPH
EVSALWFLWY VKQCGGTTRI FSVTNGGQER KFVGGSGQVS ERIMDLLGDQ VKLNHPVTHV DQSSDNIIIE
TLNHEHYECK YVINAIPPTL TAKIHFRPEL PAERNQLIQR LPMGAVIKCM MYYKEAFWKK KDYCGCMIIE
DEDAPISITL DDTKPDGSLP AIMGFILARK ADRLAKLHKE IRKKKICELY AKVLGSQEAL HPVHYEEKNW
CEEQYSGGCY TAYFPPGIMT QYGRVIRQPV GRIFFAGTET ATKWSGYMEG AVEAGERAAR EVLNGLGKVT
EKDIWVQEPE SKDVPAVEIT HTFWERNLPS.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
