Catalogue number
ANT-049
Type
Mouse Antibody Monoclonal.
Clone
YR-BrdU.
Immunogen
5-iodouridine covalently coupled to KLH.
Ig Subclass
mouse IgG1
Purification Method
Protein A column
Formulation
1mg/ml in PBS (after reconstitution).
Shipping Conditions
Antibody is shipped lyophilized at ambient temperature.
Storage Procedures
In lyophilized form, for long periods, store at 4°C in a dry environment. After reconstitution,
if not intended for use within a month, aliquot and store at -20°C.
Solubility
Reconstitute with H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.
Applications
Immunohistochemistry, flow cytometry.
Titer
1:500- 1:2,000 (assay dependent)
Note
This antibody was produced in BALB/c mice. This antibody can be used to detect proliferating cells in living tissues after incubation with BrdU which will be incorporated into newly synthesized DNA of replicating cells (during the S phase of the cell cycle), in place of thymidine during DNA replication. Antibody binding requires prior denaturation of the DNA, by exposing the cells to acid or heat.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
