Catalogue number
ANT-135
Synonyms
Integrin, alpha L, CD11A, p180, alpha polypeptide, ITGAL, LFA-1, LFA1A
TypeRat Anti Mouse Monoclonal.
Introduction
ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling.
Clone
mCD11a.
Immunogen
Enriched mouse peritoneal macrophages.
Ig Subclass
Rat IgG2b.
Purification Method
Ion exchange column.
Formulation
1mg/ml in PBS (after reconstitution).
Storage Procedures
Lyophilized: store at 4oC. After reconstitution, if not intended for use within a month, aliquot and store at -20oC.
Solubility
Reconstitute with of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.
Applications
Staining and blocking antibody. For staining, use 10μl/1,000,000 cells. This antibody will block signaling through the CD11a molecule. Titer for blocking should be determined by the investigator.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY ESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
