Catalogue number
ANT-426
Synonyms
Metalloreductase STEAP1, Six-transmembrane epithelial antigen of prostate 1, STEAP1, PRSS24, STEAP, MGC19484.
Type
Mouse Anti Human Monoclonal.
Introduction
STEAP1 is principally expressed in prostate tissue, and is found to be upregulated in multiple cancer cell lines. The STEAP1 is predicted a six-transmembrane protein, and was shown to be a cell surface antigen significantly expressed at cell-cell junctions.
Clone
PJ2D2AT.
Immunogen
Anti-human STEAP1 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human STEAP1 amino acids 1-70 purified from E. coli.
Ig Subclass
Mouse IgG2b heavy chain and κ light chain.
Purification Method
STEAP1 antibody was purification by protein-G affinity chromatography.
Formulation
1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.
Storage Procedures
For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C.
Prevent freeze thaw cycles.
Stability / Shelf Life
12 months at -20°C. 1 month at 4°C.
Applications
STEAP1 antibody has been tested by ELISA, Western blot, immunofluorescence and immunohistochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot is 1:1,000~1:2,000, immunofluorescence 1:100 ~1:300 and immunohistochemistry analysis is 1:50~100. Recommended starting dilution for Western blot is 1:1,000, Immunofluorescence 1:100 and Immunohistochemistry analysis is 1:50.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
