Catalogue number
PRO-183
Synonyms
Zinc finger CCHC domain containing 17, PS1D, pNO40, HSPC251, Pnn-interacting nucleolar protein, Putative S1 RNA-binding domain protein, RP11-266K22.1, Nucleolar protein of 40 kDa, PS1D protein.
Introduction
ZCCHC17 protein interacts with PNN and associates with the 60S ribosomal subunit. Universally expressed, ZCCHC17 is localizing to the?nucleolus, and takes part in ribosome maturation and biogenesis.
Description
ZCCHC17 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 264 amino acids (1-241a.a.) and having a molecular mass of 30.0 kDa (Molecular weight on SDS-PAGE will appear higher).
ZCCHC17 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
The ZCCHC17 protein solution (0.25mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0), 100mM NaCl, 2mM DTT, 0.1mM PMSF and 20% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.?
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMNSGRPE TMENLPALYT IFQGEVAMVT DYGAFIKIPG CRKQGLVHRT HMSSCRVDKP SEIVDVGDKV WVKLIGREMK NDRIKVSLSM KVVNQGTGKD LDPNNVIIEQ EERRRRSFQD YTGQKITLEA VLNTTCKKCG CKGHFAKDCF MQPGGTKYSL IPDEEEEKEE AKSAEFEKPD PTRNPSRKRK KEKKKKKHRD RKSSDSDSSD SESDTGKRAR HTSKDSKAAK KKKKKKKHKK KHKE.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
